Methods used in our lab include:
CAM – Chorioallantoic membrane angiogenesis model is an in vivo model used to study tumor angiogenesis and metastasis.
CRISPR-Cas9 – Clustered regularly interspaced short palindromic repeats/Cas9 genome editing system can recognize and cleave specific parts of the DNA, which allows for several applications. Our focus is on generating knockouts and knockins of our proteins of interest.
ChIP – Chromatin Immunoprecipitation is used to determine whether a protein of interest is associated with specific DNA sequences in vivo.
ChIP-seq – Chromatin Immunoprecipitation Sequencing allows for the identification of the DNA sequences bound to by proteins of interest.
Co-IP – Co-Immunoprecipitation is used to study protein-protein interactions in vivo. With the use of antibodies, proteins bound to your protein of interest can be isolated using, for example, antigen recognizing beads. The acquired proteins can then be analyzed using Western Blotting.
IF – Immunofluorescence microscopy is a great method for visualizing different components of cells and tissues, such as proteins, nucleic acids, and lipids. IF utilizes fluorescent antibodies and stains to mark structures of interest, which can then be viewed in a fluorescent microscope.
MS – Mass Spectrometry measures mass-to-charge ratio of ions to help identify and quantify molecules, such as proteins, from cells and tissues.
PRO-seq – Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on is a high resolution technique for mapping genome-wide distribution of transcriptionally-engaged polymerase II, which allows for base-pair precision of changes in gene regulation.
qPCR – Real-time polymerase chain reaction combines the amplification of PCR and detection into a single step by the use of fluorescent dyes that label the PCR product. The accumulation of the different dyes is measured in real time, which allows for quantitative measurements of the PCR products.
RNA-seq – RNA Sequencing determines the transcriptome in cells by converting it into cDNA, which can then be sequenced using next-generation sequencing.
WB – Western Blotting is a standard method to detect proteins of interest from both cells and tissues. Proteins are first separated by weight using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to a membrane, which is blotted with antibodies against proteins of interest.